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2, 2 Tell us what you think of Sartorius Pipettes and Tips www.sartorius.com/lh-satisfaction Table of Contents 1. Introduction ................................................................................................................................................................................................................................................... 3 1.1 Intended Use ............................................................................…
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3, Sartorius Tacta 3 10 11 12 1 2 3 5 4 6 7 9 8 13 1. Introduction 1.1 Intended Use The Tacta® pipette is intended, designed and manufactured for dispensing liquids in a variety of applications, and to be used in combination with Sartorius Optifit Tips or SafetySpace Filter Tips. The Sartorius pipette and tip combination, fall within the scope of in-vitro diagnostics, and can be used as a diagnostic medical device in related applications. Thereby, Tacta® and Sartorius tips fulfil the relevant demands of the Directive 98/97/EC…
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4, 4 SafetySpace air gap ] 1.3 Sartorius Pipette Tips We recommend only using Sartorius pipette tips with the Tacta® pipette. Using fully compatible tips guarantees the pipette’s performance specifications and ensures pipetting accuracy and precision. Sartorius tips are made of pure virgin polypropylene and manufactured in protected cleanroom conditions. Sartorius non-filter tips are available in trays, bulk packages…
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5, 5 3. Tagging the Pipette You can use personalized tags to make each Tacta® pipette easily identifiable. NOTE: If you intend to autoclave the pipette, make sure you use suitably resistant ink. 1. Write the identifying information on the tag. 2. Attach the tag to the finger support. 4. Stands and Holders For convenience and safety, always keep the pipette upright in its holder or stand when not in use. 4.1 Installing the Pipette Holder 1. Clean the shelf surface with ethanol. 2. Remove the protective paper from the adhesive tape. 3. …
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6, Sartorius Tacta 6 4.2 Using the Carousel Stand Adapter The Carousel Stand (Order Code 725600) is made compatible with Tacta® by using the adapter provided in the Tacta® package. 5. Preparing the Pipette 5.1 Setting the Volume The volume to be aspirated is shown on the display. To adjust the volume, you can use the volume lock button in two different ways. We recommend that in normal use the volume lock is engaged, with the red bar hidden. This prevents accidental volume changes. To adjust the volume: 1. Press and hold the volume lock bu…
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7, 7 ! Always lock after setting the volume 5.2 Attaching and Ejecting Tips The pipette has a spring-loaded tip cones for optimal sealing. To attach a tip: 1. Make sure the pipette tip cones are clean and undamaged. 2. Press the tip cone carefully into a tip held in a tip tray. If the volume is unlocked (the red bar is visible): 1. Turn the operating button to adjust the volume. 2. Slide the volume lock button down. Volume adjustment is now lo…
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8, Sartorius Tacta 8 NOTE: Do not force the pipette into the tip, or rock it from side to side, or front to back when attaching tips.
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9, 9 To eject a tip: 1. Hold the pipette over a suitable waste container. 2. Press the tip ejector with your thumb.
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10, 10 5.3 Using Protective Tip Cone Filters Replaceable Sartorius Safe-Cone Filters can be used with your pipette to help prevent liquids and aerosols from entering the pipette housing, and causing contamination or damage. We recommend you always use these filters when using non-filter tips. The operating button allows safe and easy filter ejection. NOTE: Do not use a Safe-Cone Filter with a filter tip. Safe-Cone Filters are available in Standard or Plus v…
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11, 11 0 1 2 6. Operating the Pipette The Tacta® pipette is generally used for forward and reverse pipetting. 6.1 Good Pipetting Practices − Make sure the tip is firmly attached to the tip cone(s). − Pre-rinse the tip before aspirating by filling and emptying the tip three to five times. This is particularly important when pipetting liquids with a viscosity and density greater than water, or volatile liquids with high vapor …
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12, Sartorius Tacta 12 0 1 2 6.3 Reverse Pipetting Reverse pipetting is recommended for highly viscous, biological, or foaming liquids, or very small volumes. The selected volume is aspirated into the tip along with an excess volume. Delivery is done without blow-out ensuring the excess volume remains in the tip. This excess is then discarded after the selected volume is aspirated. You may need to adjust the pipette in order to use the reve…
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13, 13 1 2 3 4 1 2 3 4 1 3 5 5 2 4 5 7.1 Cleaning the Outer Surface of the Pipette (daily) Your Tacta® pipette should be checked daily to ensure it is clean. To clean and decontaminate the outer surface of the pipette, use a disinfectant liquid or mild detergent and a soft, lint-free cloth. Gently clean the surface of the pipette with the moistened cloth and wipe it dry. Pay special attention to the tip cone. Change the tip cone filter. 7.2 Cleaning the Lower Part of the Pipette (every …
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14, Sartorius Tacta 14 c. With a 10 ml pipette hold the tip cone cylinder firmly and turn the locking ring (2) clockwise with your fingers (see image below). Do not turn the tip cone (3) as this will break the pipette. 4. Clean the tip ejector collar (1), the tip cone holder, the tip cone cylinder, and the piston (4) with a disinfectant liquid or mild detergent and a soft, lint-free cloth. 5. Clean the interior of the tip ejector collar (1) and the tip cone cylinder with a cotton…
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15, 15 To reassemble the pipette: 1. Depending on the pipette volume: a. With a 3, 10, 20, 100, 200, or 1000 μl pipette, carefully place the tip cone (3) on the piston (4) and attach it by screwing the tip cone holder clockwise. OR b. With a 5000 μl pipette, carefully place the tip cone cylinder on the piston (4) and screw clockwise. Make sure the tip cone cylinder is properly tightened. Avoid over-tightening. OR c. With a 10 ml pipette, carefully place the tip…
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16, 16 8.1 Testing Pipette Performance Performance testing should take place in a draft-free room at 15-30°C, constant to +/- 0.5°C, and with humidity above 50%. The pipette, tips, and test water should stay in the test room long enough (at least two hours) to reach equilibrium with the room conditions. Use distilled or deionized water (grade 3, ISO 3696) and an analytical balance with a readability of 0.01 mg (ISO 8655-6). Weighing 1. Adjust the desired test volume (V …
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17, Sartorius Tacta 17 8.2 Adjusting the Pipette Your Tacta® pipette has been factory-checked using the forward pipetting technique and certified at 22°C using grade-3 distilled water according to ISO 3696. The calibration is based on ISO 8655-6: Gravimetric test method for volumetric instruments. In the following situations factory calibration is not applicable and the pipette will need adjusting: − When pipetting liquids which differ from water in dens…
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18, 18 9. Troubleshooting Table 3: Symptoms and solutions Symptom Possible cause Solution Droplets left inside tip - Unsuitable tip - Use original Sartorius tips Leakage Pipetted volume too small - Pre-rinsing of the tip unsuccessful - Pre-rinse the tip again - Tip incorrectly attached - Attach tip firmly - Unsuitable tip - Use original Sartorius tips - Foreign particles between the tip and cone - Clean tip cone, attach new tip - Ti…
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19, 19 10. Order Information Order Information and Specifications These specifications are only guaranteed when original Sartorius tips are used. The specifications should be used as guidelines when establishing your own performance specifications. Table 4: Tacta® pipette specifications Order Code Channels Volume Range (μl) Colur Coding Increment (μl) Test Volume (μl) Systematic Error* (%) Random Error* (%) Safe-Cone Filters Standard Plus LH-729010 1 0.1-3 0.002 3 1.5 0.…
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20, 20 11. Warranty Information The Tacta® pipette is guaranteed for two years against defective materials or workmanship. If your Tacta® pipette fails to function at any time, contact your local Sartorius representative. ANY WARRANTY WILL, HOWEVER, BE DEEMED AS VOID IF FAULT IS FOUND TO HAVE BEEN CAUSED BY MALTREATMENT, MISUSE, UNAUTHORIZED MAINTENANCE OR SERVICE, NEGLIGENCE OF REGULAR MAINTENANCE OR SERVICE, ACCIDENTAL DAMAGE, INCORRECT STORAGE, USE OF THE PRODUCT FOR OPERATIONS OUTSIDE ITS SPECIFIED L…
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22, www.sartorius.com Contact Details Sartorius Biohit Liquid Handling Oy Laippatie 1 00880 Helsinki Finland Phone +358.9.755.951 [email protected] Sartorius Lab Instruments GmbH Otto-Brenner-Strasse 20 37079 Goettingen Germany Phone +49.551.308.0 Fax +49.551.308.3289 www.sartorius.com The status of the information, specifications, and illustrations in this manual are subject to change. Sartorius reserves the right to make changes to the technology, features, specifications, and design of the equipm…
